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The custom-designed WPA. ( A ). The panel depicts the arrangement of custom array antigen proteins on the slide glass substrate. *LIMS1 was a positive control that showed positive results for all sera. *IgG was also a positive control that was loaded with a dilution series. *Mock was a negative control that did not express the target antigen protein. ( B ). Fluorescence image results after the custom array assay are shown.

Journal: Biomedicines

Article Title: Proteome-Wide Analysis of Autoantibodies in Open-Angle Glaucoma in Japanese Population: A Pilot Study

doi: 10.3390/biomedicines13030718

Figure Lengend Snippet: The custom-designed WPA. ( A ). The panel depicts the arrangement of custom array antigen proteins on the slide glass substrate. *LIMS1 was a positive control that showed positive results for all sera. *IgG was also a positive control that was loaded with a dilution series. *Mock was a negative control that did not express the target antigen protein. ( B ). Fluorescence image results after the custom array assay are shown.

Article Snippet: After washing, goat anti-human IgG (H + L) Alexa Flour ® 647 conjugate (A21445, Thermo Fisher Scientific, Waltham, MA, USA) diluted 1:1000 was added and incubated at room temperature for 1 h. After washing again, the CWPA was air-dried and fluorescence images were measured with a fluorescence imager (Typhoon FLA 9500, Cytiva, Marlborough, MA, USA).

Techniques: Positive Control, Negative Control, Fluorescence